CalipHluor

ESYA’s proprietary A DNA-based ratiometric combination reporter of lumenal pH and Ca²⁺, allowing for pH-correctable measurements of Ca²⁺. It can target the endosome and lysosome in-vitro in both skin fibroblasts and C. elegans. It is relevant to several diseases such as Mucolipidosis, Batten’s disease, Alzheimer’s and Kufor Rakeb syndrome. In this figure (Narayanaswamy et al., Nat. Methods, 2018), representative pH and Ca²⁺ maps of early endosomes, late endosomes, and lysosomes labelled with CalipHluor are shown.